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Amino Acids and Peptides

簡要描述：The column is suitable for the separation of derivatives of amino acids （A） and peptides （P）. The common procedures are summarized in the following table. If samples are not often analyzed, esp. DABSY

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更新時間：2024-08-07
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Amino Acid and Peptide Analysis

UltraSep ESD AP

The column is suitable for the separation of derivatives of amino acids (A) and peptides (P). The common procedures are summarized in the following table. If samples are not often analyzed, esp. DABSYLchloride shows advantages.

Comparison of different procedures for derivatization
Procedure	Detection	Limit of detection	Remarks
DABS	Absorption 436 nm	< 1 pmol	Stable derivatives; primary and secondary amino acids can be determined
OPA	Fluorescence 330/440 nm	< 1 pmol	Pro, Hypro and Cys cannot be determined
FMOC	Fluorescence 265/315 nm	< 1 pmol	His and Cys will be detected only in part

DABS = DABSYLchloride (Dimethylaminoazobenzenesulfonylchloride)
OPA = o-Phthalaldehyde
FMOC = 9-Fluorenylmethoxycarbonylchloride

Examples of Analyzed Compounds	Analyzed in Application	Go to
Alanine (Ala)	AA
Arginine (Arg)	AA	AA
Aspartic acid (Asp)	AA
Cystine (Cys2)	AA	EPHARM6
Glutamic acid (Glu)	AA
Glycine (Gly)	AA
Glutathione	EPHARM6
Histidine (His)	AA
Isoleucine (Ile)	AA
Leucine (Leu)	AA
Lysine (Lys)	AA
Methionine (Met)	AA
Ornithine (Orn)	AA
Phenylalanine (Phe)	AA
Proline (Pro)	AA
Serine (Ser)	AA
Threonine (Thr)	AA
Tyrosine (Tyr)	AA
Valine (Val)	AA

COLUMNS and PRICES (€)
	Column size (mm)
	250 x 4	250 x 3	250 x 2	125 x 4	125 x 3	125 x 2
UltraSep ESD AP	360.00	340.00	355.00	320.00	295.00	290.00
	Size (mm)
	10 x 4	10 x 3	10 x 2
Precolumn cartridges (Price per pack = 5 pieces)	170.00	170.00	170.00